A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

In the realm of genome editing, LNPs have emerged as a potent vehicle for delivering CRISPR/Cas components ... interfering RNA (siRNA), mRNA (mRNA), single-guide RNA (sgRNA), and plasmid DNA (pDNA).

To construct plasmid pcDNA3.1-GFP-SY1, the GFP fragment and the SY1 ... Generation of HEK293t SPTLC2 knockout cells by CRISPR/Cas9 For the generation of SPTLC2 knockout cell line, guide RNA sequences ...

Department of Chemical and Biomolecular Engineering (BK21 Four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea ...

The T cells are activated with cytokines to stimulate cell division, and a viral vector is then used to engineer the cells to express a chimeric antigen receptor construct that binds ... such as TALEN ...

Shares of CRISPR Therapeutics CRSP have lost more than 30% in the past year. Though the stock soared at the onset of 2024, thanks to the FDA approval of its one-shot gene therapy Casgevy for two ...

It's clear though that these companies need time. Even with the only approved CAS9 based treatment out there, CRISPR is still years away from achieving its potential. In a recent update to ...

CRISPR-Cas9 is one of the biggest discoveries of the 21st century. Since it was developed in 2012, this gene-editing tool has revolutionized biology research, making it easier to study disease and ...

Here, we introduce CRISPR-Enabled Autonomous Transposable Element (CREATE), a genome editing system that combines the programmability and precision of CRISPR/Cas9 with the RNA-mediated ... original 17 ...