Upon entering the cell, in vitro or in vivo, the linkers are broken and the particles disintegrate for delivery of contents and translation of mRNA into active enzyme for CRISPR/Cas9 genome ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

A year later came another major breakthrough, as two groups described 2, 3 a novel gene-editing tool called CRISPR-Cas9 and demonstrated its use in eukaryotic cells. These two reports helped start ...

Widely used as a genome editing tool, the CRISPR-Cas9 system allows researchers to precisely induce frameshift mutations in specific genes or insert foreign nucleic acid sequences into a cell’s DNA ..

Genome-wide association studies (GWASs) have linked genetic variants to neuropsychiatric disorders, but their regulatory ...

Gene-editing tools such as CRISPR/Cas9 can be used to create isogenic cell lines, which can be further used to model a specific patient population. An isogenic cell line was created to model ...

Northwestern Medicine investigators have developed a new avenue to reprogram compromised immune cells to act against tumors, ...

The second tool is the CRISPR-Cas9 system, which allows easy and precise editing of any region of the genome. When it comes to traditional immortalized cell lines, such as HeLa or HEK293, cutting with ...

CRISPR technology has radically transformed genetic research, and at the forefront of this transformation is CRISPR screening ...

Experts hailed exciting news for some people with sickle cell disease in England as the health watchdog decided to grant ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Traditionally, CRISPR-Cas9 has been the predominant system for gene ... as Western blotting to assess gene disruptions in tumors, and in vitro screening was conducted in lymphoma cells treated with ...