Gene editing promised a revolution, but biotech layoffs, stock slumps, and industry struggles reveal a stark reality: CRISPR ...

Genome editing with various CRISPR-Cas molecule complexes ... The latter requires a copyable template for repair to accurately rejoin the DNA at the cut site. The slow variant is called homology ...

I hypothesized that mechanisms of DNA repair may create a circle of viral DNA that is more resistant to [degradation],” Lai said. To test this idea, Lai and his colleagues treated HIV infected cells ...

introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, including off-target cutting. Over the years, scientists have ...

Once a cut is made, it is a multistep, imperfect process of cellular DNA repair. CRISPR can be used to make other types of edits too, such as increasing or decreasing the effects of a gene ...

that can repair any damage caused by CRISPR-Cas systems. Defense systems that the bacteria uses to protect itself from foreign DNA often co-localize within defense islands (genomic segments that ...

It’s a new way to create “bi-paternal” mice that can survive to adulthood—but human applications are still a long way off.

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

Bring up germline editing, and most scientists cringe. The idea behind the notorious CRISPR-baby scandal, editing reproductive cells or embryos tinkers with DNA far beyond just the patient—any changes ...

The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell. After that, the next step in CRISPR gene editing is usually to alter that piece of DNA. However ...

Then Crispr came along—the elegant enzymatic apparatus that allows delivery of DNA scissors to a specific target in the genome. In December 2023, the FDA approved the first Crispr-based therapy ...