CRISPR technology has radically transformed genetic research, and at the forefront of this transformation is CRISPR screening ...

New gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), ...

To obtain high densities of sgRNA that would give signals significantly above the intracellular fluorescent background, especially when the chromatin is in open state during G and S phases ...

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.

Cell Biol. 22, 563-579. Our group has determined the crystal structure of the complex formed by anti-CRISPR protein AcrIIA4 and sgRNA-bound type II Cas9. We show that AcrIIA4 preferentially targets ...

Researchers have been working to find CRISPR/Cas nucleases with greater specificity ... related to hydrogen bonding with target DNA-sgRNA. The GFP reporting system was then used to test the ...

With a multidisciplinary team in his laboratory at the University of California, San Diego, he now develops tools through genetic engineering techniques such as CRISPR to solve the world’s insect ...