A study finds that CRISPR-Nickase, which changes just one allele of a given gene, improves gene editing efficiency compared with CRISPR-Cas9. A new method has allowed researchers to conduct the first ...

Get Instant Summarized Text (Gist) CRISPR nucleases, Cas9 and Cas12, have been engineered to evade immune detection, addressing a key challenge in CRISPR-based therapies. By identifying and ...

Here, we introduce CRISPR-Enabled Autonomous Transposable Element (CREATE ... CREATE employs a modified L1 mRNA to carry a payload gene, and a Cas9 nickase to facilitate targeted editing by ...

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.

First, in CRISPR-Cas9 technology, multiple genomic loci can be targeted simultaneously using multiple complementary sgRNAs.6–8 Second, retargeting of ZFN and TALEN requires laborious processes of ...