CRISPR-guided endonucleases were an obvious route, but for RNA, the options were few and imperfect. Cas13 proved useful for RNA knockdown, but it caused collateral damage to non-target RNA. Although ...

15 Both technologies are based on the CRISPR-Cas system but utilize different CRISPR-associated proteins, Cas13 for SHERLOCK and Cas12a for DETECTR, to achieve their highly specific diagnostic ...

CRISPR-Cas systems have been exploited for targeted genome editing. CRISPR-Cas13 mediated editing of RNA corrects a common mutation in the retinal degeneration gene USH2A, and restores usherin ...

“Prior to CRISPR, RNAi was the holy grail with respect to ways to modulate gene expression,” notes Mitchell O’Connell, who works on RNA-targeting CRISPR at the University of Rochester and who was not ...