CRISPR gene editing and next-generation sequencing (NGS ... As in the early days of immunosorbent assays or fluorescence in situ hybridization, CRISPR and NGS present significant opportunities ...

The researchers from Bonn have now developed an optical CRISPR screening method that allows identification of important genes much more easily and quickly: Nuclear In-Situ Sequencing, or NIS-Seq ...

As sequencing technology becomes ubiquitous, scientists take their research to new heights by implementing novel methods. Download this ebook from The Scientist to learn about Pairing CRISPR screening ...

Visualizing cells after editing specific genes can help scientists learn new details about the function of those genes. But ...

However, CRISPR has also been adapted to do other things too, such as turning genes on or off without altering their sequence. There were ways to edit the genomes of some plants and animals before ...

To illustrate the feasibility of targeted sequencing in conjunction with CRISPR screens, a single-cell RNA sequencing experiment was conducted using a WTA approach. Here, all expressed genes are ...

However, accurate prediction of CRISPR-Cas9 off-target sites and activity is challenging. We have developed a new protocol for detection of gRNA binding and Cas9 cleavage, based on amplification-free ...

Results prove that inversions can be generated in plants without causing further unwanted changes in the expression of genetic information.

Analysis of CRISPR/Cas9 edited cells is done to check the efficacy of the system in introducing mutations (insertions, deletions, or substitutions) in the DNA sequences of the edited cell population.