I hypothesized that mechanisms of DNA repair may create a circle of viral DNA that is more resistant to [degradation],” Lai said. To test this idea, Lai and his colleagues treated HIV infected cells ...

introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, including off-target cutting. Over the years, scientists have ...

Then, Crispr runs a seek-and-destroy function. After that, other molecules are dispatched to repair the gene with new, healthy DNA. And just like that, you can edit the human genome. But while ...

Once a cut is made, it is a multistep, imperfect process of cellular DNA repair. CRISPR can be used to make other types of edits too, such as increasing or decreasing the effects of a gene ...

However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient. Yet, inserting DNA is crucial to engineering CAR T cells.

that can repair any damage caused by CRISPR-Cas systems. Defense systems that the bacteria uses to protect itself from foreign DNA often co-localize within defense islands (genomic segments that ...

Genome editing with various CRISPR-Cas molecule complexes ... The latter requires a copyable template for repair to accurately rejoin the DNA at the cut site. The slow variant is called homology ...

Bring up germline editing, and most scientists cringe. The idea behind the notorious CRISPR-baby scandal, editing reproductive cells or embryos tinkers with DNA far beyond just the patient—any changes ...

The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell. After that, the next step in CRISPR gene editing is usually to alter that piece of DNA. However ...

Results prove that inversions can be generated in plants without causing further unwanted changes in the expression of genetic information.

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...