signaling the system to initiate gene silencing. "The 'R' in R-loop stands for RNA. All DNA-binding CRISPR-Cas systems use this structure to probe the DNA sequence and identify the correct target ...

CRISPR-Cas systems are prokaryotic immune systems ... Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

The epigenetic state of chromatin, gene activity, and chromosomal positions are interrelated. A research team from the IPK Leibniz Institute (IPK) and the Karlsruhe Institute of Technology (KIT) ...

Yet others, called base editors, change one letter of the DNA code to another. So why do we call it CRISPR? Cas proteins are used by bacteria to destroy viral DNA. They add bits of viral DNA to ...

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

The core components of CRISPR ... the immune system recognized. To find out, Zhang's team used a specialized type of mass spectrometry to identify and analyze the Cas9 and Cas 12 protein fragments ...

Complementary discoveries have the potential to enhance treatment options for antibiotic-resistant infections. Researchers at ...

Australian researchers have successfully introduced an improved version of Cas12a gene-editing enzyme in mice.

NanoCas, an ultracompact nuclease, enables efficient muscle editing in NHPs using a single AAV - advancing gene editing for hard-to-target tissues.

Results prove that inversions can be generated in plants without causing further unwanted changes in the expression of genetic information.