CRISPR technology has radically transformed genetic research, and at the forefront of this transformation is CRISPR screening ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient. Yet, inserting DNA is crucial to engineering CAR T cells.

University of California San Diego researchers have developed a new genetic system to test and analyze the underlying mechanisms of CRISPR-based DNA repair outcomes. As described in Nature ...

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

CRISPR-Cas tools allow researchers to modify ... The cut activates two natural repair mechanisms that the cell uses to repair such damage: a fast but imprecise one that reconnects only the ends ...

9 After characterizing CRISPR’s role as a defense mechanism in bacteria ... instructions for one or more Cas effectors for making the genome breaks, and repair templates in case researchers want to ...

Going far beyond programmable genetic scissors like CRISPR, the bridge recombinase mechanism enables scientists to specify not only the target DNA to be modified, but also the donor material to be ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

RNAi and CRISPR rely on the same basic biological mechanism involving non-coding ... to target and insert DNA without needing any DNA repair. The system comes from the insertion sequence 110 ...