A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

In particular, we have identified base-specific recognition of the PAM sequence and the positioning of ... crystal structure of the complex formed by anti-CRISPR protein AcrIIA4 and sgRNA-bound type ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search. When the CRISPR Cas9 protein is added to ...

Mammalian cell gene editing has extensively used RNA-guided CRISPR-Cas systems ... showed that it could identify the WTN (W = T or A) PAM sequence, greatly expanding its targeting range.

Traditionally, CRISPR-Cas9 has been the predominant system for gene editing, but limitations such as sequence constraints and off-target effects have driven the search for alternative tools.