W.M. Keck Career Development Professor of Biomedical Engineering Assistant Profes The Cas9 endonuclease from the microbial adaptive immune system CRISPR can be easily programmed to bind or cleave ...

the CRISPR-Cas9 system allows researchers to precisely induce frameshift mutations in specific genes or insert foreign nucleic acid sequences into a cell’s DNA. Scientists employ these knockout or ...

Base editing (BE) is a CRISPR-Cas9-based genome editing technology that allows the ... guide RNA (pegRNA)-guided reverse transcriptase instead of a deaminase. The development of PE was a breakthrough ...

The most well-known LNP cargo is mRNA, the key constituent of some of the early vaccines against COVID-19. But that is just one application ... is an LNP-based CRISPR-Cas9 candidate treatment ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

In the article, my colleague Jef Akst highlighted Doudna, Charpentier, and Zhang as the three seminal figures in the development of CRISPR/Cas9 technology: “The attendees are a veritable who’s who of ...

With vasa-Cas9, high efficiency and self-limiting suppression was achieved. Applying the CRISPR-Cas9 system to ... in the cascade driving fetal testis development in mice. Although compact ...