9 After characterizing CRISPR’s role as a defense mechanism in bacteria, researchers soon realized that they could harness this system for gene manipulation in any cell. All they needed to do was ...

Potent in vivo gene editing in skeletal muscle of non-human primates by a novel, ultracompact CRISPR system delivered via a single AAV vector.

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now engineered two CRISPR nucleases, Cas9 and Cas12, to mask them from the immune system. The team identified ...

Results prove that inversions can be generated in plants without causing further unwanted changes in the expression of genetic information.

Typical guide RNAs are about 20 nucleotides long for CRISPR-Cas9 tools, but the TiD system typically uses 35 ... such as Cas9, Cpf1, and Cas3. However, unlike other CRISPR systems, TiD’s ...