Optimized production and fluorescent labeling of SARS-CoV-2
WEBAug 27, 2022 · Tagging VLPs with fluorescent proteins was possible and could be very useful to image SARS-CoV-2-hACE2 internalization in host cells using single particle fluorescence quantitative...
Studying SARS-CoV-2 with Fluorescence Microscopy - PMC
WEBJun 18, 2021 · Light-sheet 3D fluorescence imaging of SARS-CoV-2-infected ferret nasal turbinates. Cyan—autofluorescence (overall tissue structure); green and magenta—staining with two different antibodies against N-protein. Only overlapping green and magenta signals are considered to be specific for SARS-CoV-2-infected cells (marked by arrows).
Imaging and visualizing SARS-CoV-2 in a new era for structural …
WEBOct 12, 2021 · A beautiful example of multiscale imaging applied to SARS-CoV-2 is the study by Bartenschlager and colleagues combining fluorescence microscopy, TEM, SEM and cryo-ET image reconstruction to visualize the ultrastructural changes in cellular structures induced by SARS-CoV-2 infection.
Imaging Techniques: Essential Tools for the Study of SARS-CoV-2 ...
WEBJul 22, 2022 · This review aims to present the up-to-date microscopy observations in patients, the in vitro studies of the virus and viral proteins and their interaction with their host, discuss the microscopy techniques for detection and study of SARS-CoV-2, and summarize the reagents used for SARS-CoV-2 detection.
Virus morphology: Insights from super-resolution fluorescence microscopy
WEBApr 4, 2022 · SMLM methods offer the highest spatial resolution to date and are typically based on relatively low-cost total internal reflection fluorescence (TIRF) microscopy systems, however, can require lengthy acquisition and extensive post-acquisition image processing to reconstruct image.
AI-boosted CRISPR-Cas13a and total internal reflection fluorescence ...
WEBOct 10, 2022 · Here, we construct a fluorescent image dataset and develop an AI-boosted CRISPR-Cas13a and total internal reflection fluorescence microscopy system for the rapid diagnosis of SARS-CoV-2.
A spatial multi-scale fluorescence microscopy toolbox ... - PubMed
WEBWe exploited a multi-scale microscopy imaging toolbox to address some major issues related to SARS-CoV-2 interactions with host cells. Our approach harnesses both conventional and super-resolution fluorescence microscopy and easily matches the spatial scale of single-virus/cell checkpoints.
Label-free SARS-CoV-2 detection and classification using phase …
WEBSep 1, 2021 · Rapid label-free detection of SARS-CoV-2 using phase imaging (spatial light-interference microscopy (SLIM)) with computational specificity.
Correlative multi-scale cryo-imaging unveils SARS-CoV-2 ... - Nature
WEBJul 30, 2021 · To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to...
COVID-19 Virus Under the Microscope | Microscope World Blog
WEBThis scanning electron microscope image shows SARS-CoV-2 (in yellow), the virus that causes COVID-19 isolated from a patient in the United States, emerging from the surface of cells (blue and pink) cultured in the lab.
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